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Sequence No.


Explain the significance of palindromic nucleotide sequences in r - DNA technology.
What is the relation between restriction endonuclease and palindromic nucleotide sequence?



Palindromic nucleotide sequences on DNA read same either we read from 5' to 3' or 3' to 5' direction. This means restriction enzymes read them from both sides which increase the chance of action of restriction enzyme to cut DNA strand. The recognition site of restriction endonuclease is a far away from the center of palindromic sites, but they cut b/w the same base/pairs of DNA strand and leaves stricky overhang end for the action of DNA ligase so presence of palindromic site promote the action of restriction endo - nucleas for the formation of r - DNA.

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