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Sequence No.


Describe the process of amplification of gene of interest PCR technique.
Describe the roll and applications of PCR.



The method which is used in r - DNA technology for the amplification of gene is polymerase chain reaction. By using sevral products such as DNA sample, primers, nucleotide, Taq polymerase, PCR manipulate the gene of interest as well as vector DNA also. Application of PCR:

  1. Diagnosis of genetic disease,
  2. used in medical diagnostics,
  3. DNA fingerprinting,
  4. Forensic analysis of DNA,
  5. Manipulation of piece of DNA.
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