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Sequence No.

BIO-752

How does EcoRI specifically act on DNA molecule? define EcoRI

 

Answer:

EcoRI is a type - 2 restriction enzyme found in E.coli It reads usually a palindromic site. That is GAATTC, where the enzyme cut G↓AATTC to create 4 nucleotide overhang of AATT with 5' end. EcoRI cut compliantly sequence of CTTAA↓G to create overhangs or sticky ends.

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