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Sequence No.


Why must bacterial cells be first made ‘Competent in r - DNA technology’?
How the insertion of recombinant DNA has to be done in host cell?



Competent is the ability to being capable to take up the recombinant DNA in it. Many types of cells are not able to take up foreing DNA, untill they have been treated with some special chamical treatments to be competent. Most probably the cells are treated with cations such as calcium or socked in CaCl2 solution which makes the channel open for the entry of recombinant DNA. Micro injection and gene, gum are the other methods for the insertion of recombinant DNA into the host cell.

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