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Sequence No.
BIO-467
(i) List the two methodologies which were involved in human genome project. Mention how they were used.
(ii) Expand ‘YAC’ and mention what was it used for.
Answer:
(i) Two major methodologies involved in human genome project are as follows:
- Expressed Sequence Tags (ESTs): This method focuses on identifying all the genes that are expressed as RNA.
- Sequence Annotation: This method involves the sequencing of whole set of genome (that contained all coding and non-coding sequence) and then assigning functions to the different regions in the sequence.
(ii) YAC, stands for yeast artificial chromosome. These were used as vectors in fragments of whole DNA of human were inserted and cloned.
Reporting Question Sequence No.
BIO-467
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