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Sequence No.


Why must a ceil he made competent in biotechnology experiments? How does calcium ion help in doing so?



Competent is the ability to being able to take up the recombinant DNA in it. Recombinant DNA can’t pass the cell membrane directly so first we need to break the cell wall and then cells are trated with cations such as calcium of soaked in CaCl2, which open the channels on the cell membfane and allow the entry of recombinant DNA into cell.

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